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한국인의 임플란트 주위 질환에서 real time PCR법을 이용한 치주세균 평가: pilot study
Evaluation of periopathogenic bacteria of peri-implantitis using real-time PCR in Koreans: pilot study
J Dent Rehabil Appl Sci 2018;34(3):186-195
Published online September 30, 2018
© 2018 Korean Academy of Stomatognathic Function and Occlusion.

이문영1, 권은영2, 김현주1, 이주연1, 주지영1*
Mun-Young Lee1, Eun-Young Kwon2, Hyun-Joo Kim1, Ju-Youn Lee1, Ji-Young Joo1*

1부산대학교 치의학전문대학원 치주과학교실
2부산대학교병원 치과진료센터

1Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University, Yangsan, Republic of Korea
2Dental Clinic Center, Pusan National University Hospital, Pusan, Republic of Korea
Ji-Young Joo
Assistant Professor, Department of Periodontology, School of Dentistry, Pusan National University, Dental Research Institute, 20 Geumo-ro, Beomeo-ri, Mulgeum-eup, Yangsan, 50612, Republic of Korea
Tel: +82-55-360-5203, Fax: 82-55-360-5194, E-mail: joojy@pusan.ac.kr
Received July 9, 2018; Revised July 26, 2018; Accepted August 6, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
목적: 한국인에서 임플란트 주위 질환의 심도에 따른 미생물학적 차이를 알아보기 위해 real time Polymerase Chain Reaction (real-time PCR)법을 이용하여 5종의 치주세균의 정량적, 정성적 분석을 시행하였다.
연구 재료 및 방법: 임플란트가 식립된 총 60명의 환자를 치근단 방사선 사진 및 임상지수 검사를 통해 3군(건강군, 임플란트 주위 점막염군, 임플란트 주위염군)으로 나누었다. 멸균된 curette기구를 이용해 치은연하에서 미생물 샘플을 채취한 후 치주세균 5종에 관해 real time PCR을 시행하였고 comparative delta-CT method를 이용하여 분석한 후 미생물의 상대적 발현량을 비교하였다.
결과: Eikenella corrodens, Treponema denticola의 상대적 발현량은 임플란트 주위염 그룹에서 유의하게 높게 나타났다(P < 0.017). 반면 Fusobacterium nucleatum, Porphyromonas gingivalis의 상대적 발현량은 질환의 심도와는 관련 없이 건강한 임플란트 그룹에서 가장 높게 나타났다. Prevotella intermedia의 상대적 발현량은 건강한 임플란트 그룹에서 유의하게 낮게 나타났다(P < 0.017).
결론: 한국인의 임플란트 주위질환에서 대표적인 치주염 세균이 검출되었으나 치주염과 유사한 미생물학적 분포를 보이지는 않았다.
Purpose: We performed quantitative and qualitative analysis of typical periodontal bacteria using real time PCR method to investigate the microbiological difference according to the severity of peri-implant disease in Koreans.
Materials and Methods: Total of 60 implants were divided into three groups (healthy group, peri-implant mucositis group, peri-implantitis group) through periapical radiographs and clinical indices. The evaluated clinical parameters were pocket depth, plaque index, suppuration and bleeding on probing. Using a sterilized curette instrument, microbial samples were collected from the subgingival plaque and real-time PCR was performed on five periodontal bacteria. The relative expression levels of microorganisms were compared by comparative delta- CT method.
Results: The relative expression levels of E. corrodens and T. denticola were significantly higher in the peri-implantitis group (P < 0.017). On the other hand, the relative expression level of F. nucleatum and P. gingivalis was relatively high in the healthy implant group regardless of the severity of disease. P. intermedia was significantly lower in the healthy implant group (P < 0.017).
Conclusion: Periodontal bacteria were detected in Koreans with peri-implant diseases, but there was no microbiological distribution similar to periodontitis.
주요어 : 임플란트 주위염; 미생물; 한국인; 예비연구
Keywords : peri-implantitis; microbiota; Koreans; pilot study


September 2018, 34 (3)
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