Fig. 3. Expression of EGFR, CTGFR, TGFBR1, TGFBR2, MEK2, ERK1, ERK2, c-Fos, JNK1, JNK2, PAK1, and PEBP1 gene of human gingival fibroblasts cultured on smooth (NE0), acid-etched (E0), microgrooved (E60/10), and fibronectin-immobilized (NE0FN, E0FN, and E60/10FN) titanium substrata after 48 and 72 h of culture using semi quantitative real-time RT-PCR. The relative expression levels were analyzed by normalizing with GAPDH, and are presented as fold changes relative to the control, NE0 at 48 h of culture. One-way ANOVA (n = 5). ***: significant difference (P < 0.001). **: significant difference (P < 0.01). *: significant difference (P < 0.05). Note that only significant differences between the highest gene expression levels and other gene expression levels are presented.
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