Fig. 2. Expression of AKT1, AKT2, PIK3CG, PRKAR1A, NF-κB, CREB1, and CREB2 gene of human gingival fibroblasts cultured on smooth (NE0), acid-etched (E0), microgrooved (E60/10), and fibronectin-immobilized (NE0FN, E0FN, and E60/10FN) titanium substrata after 48 and 72 h of culture using semi quantitative real-time RT-PCR. The relative expression levels were analyzed by normalizing with GAPDH, and are presented as fold changes relative to the control, NE0 at 48 h of culture. One-way ANOVA (n = 5). ***: significant difference (P < 0.001). **: significant difference (P < 0.01). Note that only significant differences between the highest gene expression levels and other gene expression levels are presented.
© 2022 J Dent Rehabil Appl Sci
